The ambition of this project is to design a new integrated process for the production of second generation sugars (2GS) using ligninolytic enzymes (LE) and novel cellulases (CA). Basis for the generation of 2GS is the organosolv process which allows the fractionation of lignocellulosic material into cellulose, hemicellulose and lignin. This is carried out under relatively severe conditions and requires the use of energy, chemicals and specialized equipment. The resulting cellulose is hydrolyzed enzymatically to glucose. The innovative idea lies in the combined application of CA and LE in both steps of the 2GS production, leading to a reduced consumption of energy and chemicals and higher yields of fermentable sugars.